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Looking for key publications about single-cell technology? 

Here you have some.

Tutorial: guidelines for the experimental design of single-cell RNA sequencing studies

Lafzi, A., et al. Nat Protoc. 13, 2742–2757 (2018).

Combinatorial single-cell CRISPR screens by direct guide RNA capture and targeted sequencing.

Replogle, J.M., et al. Nat Biotechnol. 38, 954–961 (2020).

Benchmarking single-cell RNA-sequencing protocols for cell atlas projects.

 Mereu, E., et al. Nat Biotechnol. 38, 747–755 (2020).

Dissociation of solid tumor tissues with cold active protease for single-cell RNA-seq minimizes conserved collagenase-associated stress responses. 

O'Flanagan CH, et al. Genome Biol. 2019 Oct 17;20(1):210. doi: 10.1186/s13059-019-1830-0.

Cell Hashing with barcoded antibodies enables multiplexing and doublet detection for single cell genomics.

 Stoeckius, M., et al. Genome Biol. 19, 224 (2018).

Sampling time-dependent artifacts in single-cell genomics studies.

Massoni-Badosa, et al. Genome Biol. 21, 112 (2020).

Large-scale simultaneous measurement of epitopes and transcriptomes in single cells

 Stoeckius M, et al. Nat Methods. 14(9):865-868 (2017). doi:10.1038/nmeth.4380

Systematic assessment of tissue dissociation and storage biases in single-cell and single-nucleus RNA-seq workflows.

Denisenko E. et al. Genome Biol. Jun 2;21(1):130 (2020). doi: 10.1186/s13059-020-02048-6.

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